meta-analysis for cross-platformand cross-species comparisons
Dear all, I am trying to analyze the expression of a small set of genes across different platforms and different bacterial species using publicly-available microarray and RNAseq data...should I use JASP with the meta-analysis module? any idea which would be the best pipeline? As far as I know, we have two potential approaches...1) calculate logFC data for the genes of interest in each platform and then normalize them, and 2) normalize raw data and then evaluate logFC for each gene...any thoughts?. Suggestions are wellcome. Cheers!
Comments
That sounds like an interesting approach. I am not a geneticist so I don't know what normalization is required or what would be the best way.
Cheers,
E.J.